Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species

A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.

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Author:Petar Stupar, Vladimir B. Pavlović, Jana Nunić, Sandra Cundrič, Metka Filipič, Magdalena Stevanović
Parent Title (English):Journal of Drug Delivery Science and Technology
Publisher:Association de pharmacie galenique industrielle
Place of publication:Paris
Document Type:Preprint
Date of Publication (online):06.03.2014
Year of first Publication:2014
Tag:freeze drying; physicochemical solvent and non-solvent approach; poly(epsilon- caprolactone); reactive oxygen species
First Page:191
Last Page:197
The final version of the paper is published as: Stupar, P., Pavlović, V., Nunić, J., Cundrič, S., Filipič, M. & Stevanović, M. 2014, "Development of lyophilized spherical particles of poly(epsilon- caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species", Journal of Drug Delivery Science and Technology, vol. 24, no. 2, pp. 191-197. 
Institutes:Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
University of Belgrade, Faculty of Physical Chemistry
University of Belgrade, Faculty of Agriculture
National Institute of Biology, Ljubljana, Slovenia
University of Ljubljana, Biotechnical Faculty
open_access (DINI-Set):
MESTD Projects 2011-2017:III 45004 - Molecular designing of nanoparticles with controlled morphological and physico-chemical characteristics and functional materials based on them
Licence (English):License LogoCreative Commons - Attribution-Noncommercial-No Derivative Works 3.0 Serbia